Next Generation Sequencing (NGS) in Forensic Genetics and Anthropology Training Course

Introduction to NGS and its Forensic Applications

• Overview of modern DNA sequencing technologies
• Key advantages of NGS compared to traditional forensic methods
• Typical forensic use cases for NGS

Foundations of Forensic Genetics

• DNA markers frequently used in forensic analysis
• Short Tandem Repeats (STRs) and Single Nucleotide Polymorphisms (SNPs)
• Mitochondrial DNA and Y-chromosome analysis

NGS Workflows in Forensic Casework

• Sample preparation and library construction
• Sequencing protocols optimized for forensic samples
• Quality control measures and contamination prevention

Bioinformatics and NGS Data Analysis

• Processing and alignment of NGS data
• Variant calling and forensic interpretation
• Software tools for forensic genomic analysis

Forensic Anthropology and Ancestry Determination

• Genetic markers relevant to ancestry and population studies
• NGS applications in analyzing ancient and degraded DNA
• Case studies in forensic anthropology

NGS for Challenging and Degraded Samples

• Techniques for handling low-quality and low-quantity DNA
• Managing environmental and mixed DNA samples
• NGS applications in identifying missing persons and disaster victims

Legal, Ethical, and Regulatory Considerations

• Privacy concerns within forensic genomics
• Regulatory frameworks governing forensic DNA databases
• Ethical challenges in forensic genetic analysis

Future Trends in Forensic Genomics

• Advancements in sequencing technology
• Integration of AI in forensic genetics
• Emerging forensic applications of NGS

Summary and Next Steps